Different modes of NF- B/Rel activation in pancreatic lobules
نویسندگان
چکیده
Algül, Hana, Yusuke Tando, Michael Beil, Christoph K. Weber, Claus Von Weyhern, Günter Schneider, Guido Adler, and Roland M. Schmid. Different modes of NFB/Rel activation in pancreatic lobules. Am J Physiol Gastrointest Liver Physiol 283: G270–G281, 2002; 10.1152/ajpgi.00407.2001.—The eukaryotic transcription factor nuclear factorB (NFB)/Rel is activated by a large variety of stimuli. It has been demonstrated that NFB/Rel is induced during the course of cerulein pancreatitis. Here, we show that NFB/Rel is differentially activated in pancreatic lobules. Cerulein induces NFB/Rel via activation of I B kinase (IKK), which causes degradation of I B but not I B . Tumor necrosis factor-mediated IKK activation leads to I B and I B degradation. In contrast, oxidative stress induced by H2O2 activates NFB/Rel independent of IKK activation and I B degradation; instead I B is phosphorylated on tyrosine. H2O2 but not cerulein-mediated NFB/Rel activation can be blocked by stabilizing microtubules with Taxol. Inhibition of tubulin polymerization with nocodazole causes NFB/Rel activation in pancreatic lobules. These results propose three different pathways of NFB/Rel activation in pancreatic acinar cells. Furthermore, these data demonstrate that microtubules play a key role in IKK-independent NFB/Rel activation following oxidative stress.
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